Journal: Frontiers in Aging Neuroscience

Article Title: Increased tau-induced inflammatory responses are associated with a greater degree of atherosclerosis in progressive supranuclear palsy

doi: 10.3389/fnagi.2025.1608631

Figure Lengend Snippet: Greater atherosclerotic burden and elevated plasma total tau, p-tau181 levels in patients with PSP. Using carotid artery Doppler ultrasound to assess the degree of carotid artery atherosclerosis by evaluating (A) max-CIMT, (B) max-CPT, (C) TPN, (D) CPS, and (E) carotid stenosis% in HCs ( n = 56) and patients with PSP ( n = 56). Using MRA to evaluate the degree of intracranial arterial atherosclerosis by recording (F) ICS vascular territory and (G) MRA GSS in HCs ( n = 56) and patients with PSP ( n = 56). (H) Heatmap of multivariable linear regression heatmap showing the association between disease duration/clinical scores H&Y staging scale, UPDRS-III, PSPRS, MMSE and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). Peripheral (I–L) blood lipid, (M) glycosylated hemoglobin, (N) homocysteine parameters, and plasma levels of (O) total tau, (P) p-tau181, (Q) p-tau396 in HCs ( n = 56) and patients with PSP ( n = 56). (R) Heatmap of multivariable linear regression heatmap showing the association between tau levels (total tau, p-tau181, p-tau396) and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). Results are expressed as mean and SD. Statistical approaches: (A–E, G, I–Q) covariance (ANCOVA), with age, gender, smoking status and stroke history as covariates, (F) chi-square test, (H, R) multivariable linear regression. In heatmaps, each cell represents the standardized regression coefficient (β) derived from multivariable linear regression models, where each Y -axis was regressed on X -axis while adjusting for age, gender, smoking history, hypertension, diabetes, and dyslipidemia (For MMSE, one more correction for period of education). The color intensity of heatmap indicates the strength and direction of association (β), with positive associations shown in red and negative in blue. P < 0.05 indicates statistical significance (* p < 0.05, ** p < 0.01, *** p < 0.001), and ns indicates p ≥ 0.05.

Article Snippet: The following ELISA kits were used: Human interleukin-6 (IL-6) ELISA kit (E-HSEL-H0003, Elabscience Biotechnology, Wuhan, China), Human interleukin-1β (IL-1β) ELISA kit (E-HSEL-H0001, Elabscience Biotechnology, Wuhan, China), Human interleukin-10 (IL-10) ELISA kit (E-EL-H0005, Elabscience Biotechnology, Wuhan, China), Human interferon γ (IFN-γ) ELISA kit (E-HSEL-H0007, Elabscience Biotechnology, Wuhan, China), Human tumor necrosis factor-α (TNF-α) ELISA kit (E-HSEL-H0109c, Elabscience Biotechnology, Wuhan, China), Human total tau ELISA kit (ml057755, mlbio, Shanghai, China),and Human P-tau181 ELISA kit (ml057691, mlbio, Shanghai, China), Human P-tau396 ELISA kit (E-EL-H5314c, Elabscience Biotechnology, Wuhan, China), Mouse interleukin-6 (IL-6) ELISA kit (ml098430, mlbio, Shanghai, China), Mouse interleukin-1β (IL-1β) ELISA kit (ml098416, mlbio, Shanghai, China) and Mouse tumor necrosis factor-α (TNF-α) ELISA kit (mIC50536-1, mlbio, Shanghai, China).

Techniques: Clinical Proteomics, Derivative Assay

Journal: Frontiers in Aging Neuroscience

Article Title: Increased tau-induced inflammatory responses are associated with a greater degree of atherosclerosis in progressive supranuclear palsy

doi: 10.3389/fnagi.2025.1608631

Figure Lengend Snippet: Elevated peripheral blood macrophage ratio and plasma inflammatory cytokine levels in patients with PSP. Representative flow cytometry analysis of (A) macrophages (CD14 + CD68+), and (B) classically activated macrophages (CD86 + CD206-M1) subpopulations from peripheral blood (left) and quantifications (right) in HCs ( n = 10) and patients with PSP ( n = 10). Plasma levels of inflammatory cytokine (C) CRP, (D) IL-6, (E) IL-1β, (F) IL-10, (G) TNF-α, and (H) IFN-γ in HCs ( n = 56) and PSP with patients ( n = 56). (I) Heatmap of multivariable linear regression heatmap showing the association between inflammatory cytokine levels (CRP, IL-6, IL-1β, IL-10, TNF-α, and IFN-r) and atherosclerosis measures (max-CIMT, max-CPT, TPN, CPS, carotid stenosis% and MRA GSS) in patients with PSP ( n = 56). (J) Heatmap of multivariable linear regression heatmap showing the association between tau levels (total tau, p-tau181, p-tau396) and inflammatory cytokine levels (CRP, IL-6, IL-1β, IL-10, TNF-α, and IFN-r) in patients with PSP ( n = 56). Results are expressed as mean and SD. Statistical approaches: (A, B) unpaired non-parametric Mann-Whitney U tests, (C–H) covariance (ANCOVA), with age, gender, smoking status and stroke history as covariates, (I, J) multivariable linear regression. In heatmaps, each cell represents the standardized regression coefficient (β) derived from multivariable linear regression models, where each Y -axis was regressed on X -axis while adjusting for age, gender, smoking history, hypertension, diabetes, and dyslipidemia. The color intensity of heatmap indicates the strength and direction of association (β), with positive associations shown in red and negative in blue. P < 0.05 indicates statistical significance (* p < 0.05, ** p < 0.01, *** p < 0.001), and ns indicates p ≥ 0.05.

Article Snippet: The following ELISA kits were used: Human interleukin-6 (IL-6) ELISA kit (E-HSEL-H0003, Elabscience Biotechnology, Wuhan, China), Human interleukin-1β (IL-1β) ELISA kit (E-HSEL-H0001, Elabscience Biotechnology, Wuhan, China), Human interleukin-10 (IL-10) ELISA kit (E-EL-H0005, Elabscience Biotechnology, Wuhan, China), Human interferon γ (IFN-γ) ELISA kit (E-HSEL-H0007, Elabscience Biotechnology, Wuhan, China), Human tumor necrosis factor-α (TNF-α) ELISA kit (E-HSEL-H0109c, Elabscience Biotechnology, Wuhan, China), Human total tau ELISA kit (ml057755, mlbio, Shanghai, China),and Human P-tau181 ELISA kit (ml057691, mlbio, Shanghai, China), Human P-tau396 ELISA kit (E-EL-H5314c, Elabscience Biotechnology, Wuhan, China), Mouse interleukin-6 (IL-6) ELISA kit (ml098430, mlbio, Shanghai, China), Mouse interleukin-1β (IL-1β) ELISA kit (ml098416, mlbio, Shanghai, China) and Mouse tumor necrosis factor-α (TNF-α) ELISA kit (mIC50536-1, mlbio, Shanghai, China).

Techniques: Clinical Proteomics, Flow Cytometry, MANN-WHITNEY, Derivative Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation

doi: 10.1111/jcmm.16275

Figure Lengend Snippet: Phase II validation: Candidate proteins evaluate disease severity and predict patient outcome. A, Concentration levels of RETN, MMP8, LCN2, HP, OLFM4, ELANE, TCN1, DEFα4, BPI and LTF in plasma obtained at the first sampling time‐point (T1) from healthy donors (n = 25) and influenza patients (n = 63) and presented as scatter diagram. HD: Healthy donors; M: Moderate patient (n = 34); S: Severe patients (n = 29). B, Severity prediction using LCN2, BPI, ELANE and MMP8 expression levels, and proportion of neutrophils and lymphocytes. Receiver‐operator characteristic (ROC) curves of four candidate proteins and two clinical indicators to predict disease severity: ROC‐AUC (LCN2: 0.646; BPI: 0.774; ELANE: 0.671; MMP8: 0.872; Proportion of neutrophils: 0.754; Proportion of Lymphocytes: 0.650). C, The protein levels of BPI, MMP8, DEFα4, OLFM4, LCN2 and ELANE in survivors (n = 49) and non‐survivors (n = 14) with influenza infection. Statistical significance is determined by unpaired t test. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The concentration levels of human resistin (RETN), human matrix metalloproteinase‐8 (MMP8), human lipocalin 2 (LCN2), human haptoglobin (HP) , human olfactomedin 4 (OLFM4), human neutrophil elastase (ELANE), human bactericidal/permeability‐increasing protein (BPI), human lactoferrin (LTF; Elabscience, Wuhan, China), human transcobalamin I (TCN1) and human defensin α4 (DEFA4) (mlbio, Shanghai, China) in the plasma samples were measured according to manufacturer's instructions, respectively.

Techniques: Concentration Assay, Sampling, Expressing, Infection